Microbial DNA concentrations from DNA extraction using Qiagen PowerSoil Pro DNA Extraction kit and subsequent Polymerase Chain Reaction (PCR) from core samples collected and preserved for microbiology (SSK105556 and SSK105560) from UKGEOS Glasgow Observatory, borehole GGC01. DNA extractions and subsequent amplification using PCR indicate levels of microbial DNA below detection limits. NERC grant SHAPE-UK NE/R018006/1.

Loan IDA number IDA271576. Microbial DNA concentrations from DNA extraction using Qiagen PowerSoil Pro DNA Extraction kit and subsequent PCR from shale and sandstone core samples collected and preserved for microbiology (shale SSK111456 and sandstone SSK111457) from UKGEOS Glasgow Observatory, borehole GGC01. DNA extractions and subsequent amplification using PCR indicate levels of microbial DNA below detection limits. Samples and data are derived from the UK Geoenergy Observatories Programme funded by the UKRI Natural Environment Research Council and delivered by the British Geological Survey.

Loan IDA number - IDA271576. No data was obtained for microbial cultivation experiments with core samples SSK111460 and SSK111461 from UKGEOS Glasgow Observatory, borehole GGC01. Samples and data are derived from the UK Geoenergy Observatories Programme funded by the UKRI Natural Environment Research Council and delivered by the British Geological Survey. Attached document described methodology of enriching sandstone core (SSK111461) in different media types and shale core (SSK111460) in synthetic groundwater. No microbial growth was seen after 7 months.